Journal: The Journal of Biological Chemistry

Article Title: Hydrogen sulfide–dependent activation of human sulfide quinone oxidoreductase

doi: 10.1016/j.jbc.2025.110681

Figure Lengend Snippet: Effect of sulfurtransferase knockdowns on SQOR activity . A – B , Western blot analysis of MPST ( A ) and TST ( B ) in control (HT-29 and OR7G3) versus the corresponding CRISPR knockdown cell lines. Ponceau staining is shown as equal loading controls. C , representative Western blot of SQOR in control HT-29 cells ( lane 1) compared with HT-29 cells with CRISPR knockdown of OR7G3 ( lane 2), MPST ( lane 3), and TST ( lane 4). The image was spliced at the position indicated by the dashed line to remove a lane that is not relevant to this study. D , SQOR activity in HT-29 cells with the indicated CRISPR knockdowns. Each data point represents an independent experiment and is the mean ± SD of at least two technical replicates. Significance was determined using a two-tailed t test. MPST, mercaptopyruvate sulfurtransferase; OR7G3, olfactory receptor 7G3; SQOR, sulfide quinone oxidoreductase; TST, thiosulfate sulfurtransferase.

Article Snippet: The SQOR (17256-1-AP; Proteintech), TST (66018-1-Ig; Proteintech Group), and MPST (Novus Biologicals; NBP154734) antibodies were purchased from the indicated vendors.

Techniques: Activity Assay, Western Blot, Control, CRISPR, Knockdown, Staining, Two Tailed Test

Journal: The Journal of Biological Chemistry

Article Title: Hydrogen sulfide–dependent activation of human sulfide quinone oxidoreductase

doi: 10.1016/j.jbc.2025.110681

Figure Lengend Snippet: Effect of cystine, PPG, and BSO on SQOR activity in HT-29 cells with MPST and TST knockdowns . A , SQOR activity in cells grown in the presence of cystine (750 μM final concentration) for 24 h. B – C , SQOR activity in cells treated for 96 h with 2.5 mM PPG ( B ) or 20 μM BSO ( C ). Each data point represents an independent experiment and is the mean ± SD of at least two technical replicates. Significance was determined using the two-tailed t test. BSO, buthionine sulfoximine; MPST, mercaptopyruvate sulfurtransferase; PPG, ropargylglycine; SQOR, sulfide quinone oxidoreductase; TST, thiosulfate sulfurtransferase.

Article Snippet: The SQOR (17256-1-AP; Proteintech), TST (66018-1-Ig; Proteintech Group), and MPST (Novus Biologicals; NBP154734) antibodies were purchased from the indicated vendors.

Techniques: Activity Assay, Concentration Assay, Two Tailed Test

Journal: Communications Medicine

Article Title: The treatment of primary CoQ deficiency requires the targeting of multiple pathogenic mechanisms

doi: 10.1038/s43856-025-01000-8

Figure Lengend Snippet: Representative image of Western blot of SQOR and the quantitation of the protein bands ( a ), and relative mRNA levels for Sqor ( b ) in the liver. c Representative image of Western blot of Eif2ak2 and the quantitation of the protein bands in the liver. Relative mRNA levels of the genes Cmpk2 ( d ), Mthfd2l ( e ), Psat1 ( f ) and Eif2ak2 ( g ) in liver. Data are expressed as mean ± SD (one-way ANOVA with a Tukey’s post hoc test; n = 5 for each group). Representative images of Western blots of SQOR ( h ), CBS ( i ), PSAT1 ( j ) and EIF2AK2 ( k ), and the quantitation of the protein bands in human skin fibroblast from control and patient 1 (P1). Relative mRNA levels of the genes CMPK2 ( l ), MTHFD2L ( m ), PSAT1 ( n ) and EIF2AK2 ( o ) in human skin fibroblast from control and patient 1 (P1). Representative images of Western blots of SQOR ( p ), CBS ( q ), PSAT1 ( r ) and EIF2AK2 ( s ), and the quantitation of the protein bands in human skin fibroblast from control and patient 2 (P2). Relative mRNA levels of the genes CMPK2 ( t ), MTHFD2L ( u ), PSAT1 ( v ) and EIF2AK2 ( w ) in in human skin fibroblast from control and patient 2 (P2). Data are expressed as mean ± SD (one-way ANOVA with a Tukey’s post hoc test; n = 3 for each group). CBS cystathionine β-synthase, CMPK2 cytidine monophosphate kinase 2, EIF2AK2 eukaryotic translation initiation factor 2 alpha kinase 2, MTHFD2L methylenetetrahydrofolate dehydrogenase (NADP + Dependent) 2-Like, PSAT1 phosphoserine aminotransferase 1, SQOR sulfide:quinone oxidoreductase. Original western-blot membranes corresponding to this figure are provided in Figs. S8 and Fig S9.

Article Snippet: The following primary antibodies were used: anti-COQ4 (Proteintech, 16654-1-AP), anti-COQ5 (Proteintech, 17453-AP), anti-COQ7 (Proteintech, 15083-1-AP), anti-PRODH (Cell Signaling, #22980), anti- SQRDL (Proteintech, 17256-1-AP), anti-CBS (Proteintech, 14787-1-AP), anti-EIF2AK2 (Proteintech, 18244-1-AP), anti-PSAT1 (Proteintech, 10501-1-AP), anti-VDAC1 (Abcam, ab14734) and anti-ACTIN (Invitrogen, #MA5-15739-HRP).

Techniques: Western Blot, Quantitation Assay, Control